Production of transgenic adult plants from clementine mandarin by enhancing cell competence for transformation and regeneration
نویسندگان
چکیده
منابع مشابه
Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.
A reproducible system for the generation of fertile, transgenic maize plants has been developed. Cells from embryogenic maize suspension cultures were transformed with the bacterial gene bar using microprojectile bombardment. Transformed calli were selected from the suspension cultures using the herbicide bialaphos. Integration of bar and activity of the enzyme phosphinothricin acetyltransferas...
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introduction lentil is an important pulse crop in iran. lentil is a seed propagating, self-pollinating crop originating from near east. it is containing 27.5% -31.75% protein and it has high level of iron, calcium, phosphor, thiamin, niacin, and some unusual amino acid as hydroxyarnitin, hydroxyarginin and homo arginin. faostat reported that the world production of lentils was 4,975,621 metric ...
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Regeneration of explants plays a significant role in plant transformation. Explant type, hormonal concentration, and pre-culturing period are important in transformation efficiency. To get an efficient transformation of canola and optimize regeneration conditions, different explants along with different culture media were studied. Four canola varieties were used to evaluate regeneration ability...
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A method for transformation of B.napus is presented in this study. The protocol is based on infection of cotyledonary petioles of 5-day-old and hypocotyl segments of 14-day-old seedlings with an Agrobacterium tumefaciens strain LBA 4404 carrying a disarmed binary vector pBI121. The T-DNA region of this binary vector contains the neomycin phosphotransferase II (NPTII) and glucuronidase (GUS) gen...
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ژورنال
عنوان ژورنال: Tree Physiology
سال: 2008
ISSN: 0829-318X,1758-4469
DOI: 10.1093/treephys/28.1.55